Tag

Part:BBa_K1381024

Designed by: Viktor Blomkvist, Tim Hagelby Edstrom, Laura Pacoste and Tersea Reinli   Group: iGEM14_Uppsala   (2014-10-07)

B0034-PgsA-(EAAAR)x4

Anchor tag to make fusion protein for cell surface display.

Anchor motif, PgsA from Bacillus Subtilis. Can be used in RFC25-assembly to make a fusion for cell surface display. The part starts with the RBS B0034 and is followed by the PgsA gene+linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1149


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Categories
Parameters
None